Technologies Delivered

Separation Technology:
Adsorptive Purification of Biomolecules developed for specific industry

Purification of Rifamycin B by expanded bed adsorption directly from fermentation solution

Purification of Penicillin G directly from fermentation broth on expanded bed column

Purification of lysozyme and Conalbumin from chicken egg white extract

Purification of lactoferrin and lactalbumin from milk or whey

Purification of tubulin from goat brain homogenate

Purification of lipoxygenase from soyabean seeds using ion-exchange resins

Purification of polyphenol oxidase from potato extract in RPC mode

Purification of trypsin from a mixture of trypsin and chymotrypsin

Purification of Whey proteins

Purification of monoclonal antibodies

Purification of Protein A

Purification of VitaminB12

Purification of carotenoids from palm oil

Purification of active pharmaceutical ingredients from natural sources

Purification of synthetic APIs

Purification of Bromelain and Peroxidase

Purification of synthetic peptides

 

Enzyme technology:

Peroxidase catalyzed oxidation of phenols

Selective Oxidase catalyzed oxidation of glucose

Peroxidase catalyzed nitration and amination of naphthols

Lipase catalyzed esterifications

Polymerization/Depolymerization using Immobilized Enzymes

 

The work done involves immobilization of enzymes like glucose oxidase, trypsin, horse radish peroxidase, lipase, amylase, pullulanase, pectinase, pepsin, neutral protease, etc. onto new polymer systems developed in our laboratory as well as supplied by Resindion srl under their sponsored project. Thus, oxidations using oxidases and peroxidases, hydrolysis, chiral resolution and esterification using lipases, hydrolysis and synthesis of peptides using proteases and depolymerization using depolymerases have also been developed. The basic idea behind all the work is to intensify the enzyme catalysed reactions by removing or minimizing the diffusional resistances present in traditional immobilized enzyme catalyzed reactions. Use of soluble ‘smart’ polymer supported enzymes and use of superporous adsorbent matrices has resulted in significant gains. Immobilized depolymerases have been used to develop cost effective technologies for the hydrolysis of natural polysaccharides to more value added products like soluble fibre and maltodextrins.

 

Processes have been developed for a few products and the technologies are being transferred to industry. These processes offer controlled and specific reactions giving high yields and pure products under mild and eco-friendly conditions with zero waste. The processes developed are scalable, and several of these are transferred or are being transferred to industries like BIOCON, Advanced Enzyme Technologies, etc.